The exopolyphosphatase gene from Sulfolobus solfataricus: Characterization of the first gene found to be involved in polyphosphate metabolism in Archaea

Cardona, ST; Chávez FP; JEREZ, CA

Abstract

Inorganic polyphosphate (polyP) polymers are widely distributed in all kinds of organisms. Although the presence of polyP in members of the domain Archaea has been described, at present nothing is known about the enzymology of polyP metabolism or the genes involved in this domain. We have cloned, sequenced, and overexpressed an exopolyphosphatase (PPX) gene (ppx) from thermophilic Sulfolobus solfataricus. The gene codes for a functional PPX and possesses an open reading frame for 417 amino acids (calculated mass, 47.9 kDa). The purified recombinant PPX was highly active, degrading long-chain polyP (700 to 800 residues) in vitro at 50 to 60°C. The putative PPXs present in known archaeal genomes showed the highest similarity to yeast PPXs. In contrast, informatic analysis revealed that the deduced amino acid sequence of S. solfataricus PPX showed the highest similarity (25 to 45%) to sequences of members of the bacterial PPXs, possessing all of their conserved motifs. To our knowledge, this is the first report of an enzyme characterized to be involved in polyP metabolism in members of the Archaea.

Más información

Título según WOS: The exopolyphosphatase gene from Sulfolobus solfataricus: Characterization of the first gene found to be involved in polyphosphate metabolism in Archaea
Título según SCOPUS: The exopolyphosphatase gene from Sulfolobus solfataricus: Characterization of the first gene found to be involved in polyphosphate metabolism in Archaea
Título de la Revista: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volumen: 68
Número: 10
Editorial: American Society for Microbiology
Fecha de publicación: 2002
Página de inicio: 4812
Página final: 4819
Idioma: English
DOI:

10.1128/AEM.68.10.4812-4819.2002

Notas: ISI, SCOPUS