The expression of genes involved in microcin maturation regulates the production of active microcin E492

Corsini, G; Baeza, M.; Monasterio O.; Lagos R.

Abstract

The production of active microcin E492, a channel-forming bacteriocin, was studied in exponential and stationary phase. The structural gene for this bacteriocin (mceA) is transcribed in exponential as well as in stationary phase, but the active form is produced only during the exponential phase of growth. An inactive form of microcin E492 was purified from the stationary phase. The production of the inactive form correlated with the lack of transcription in the stationary phase of two genes (mceIJ) involved in microcin E492 maturation, consequently behaving as the inactive form purified from mutants in these genes. The inactive form of microcin purified from the stationary phase as well as the inactive form purified from mutants in the maturation genes (mceC, I, J) were unable to compete with the active form when tested using a viability test on sensitive cells. This result strongly suggests that the inactive form of microcin caused by the lack of expression of the maturation genes is impaired at the level of receptor recognition. © 2002 Société française de biochimie et biologie moléculaire / Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

Más información

Título según WOS: The expression of genes involved in microcin maturation regulates the production of active microcin E492
Título según SCOPUS: The expression of genes involved in microcin maturation regulates the production of active microcin E492
Título de la Revista: BIOCHIMIE
Volumen: 84
Número: 05-jun
Editorial: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Fecha de publicación: 2002
Página de inicio: 539
Página final: 544
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0300908402014153
DOI:

10.1016/S0300-9084(02)01415-3

Notas: ISI, SCOPUS