Mechanisms of ammonium transport, accumulation, and retention in ooyctes and yeast cells expressing Arabidopsis AtAMT1; 1

Wood, Craig C.; Poree, Fabien; Dreyer, Ingo; Koehler, Gabriele J.; Udvardi, Michael K.


Ammonium is a primary source of N for plants, so knowing how it is transported, stored, and assimilated in plant cells is important for rational approaches to optimise N-use in agriculture. Electrophysiological studies of Arabidopsis AtAMT1;1 expressed in oocytes revealed passive, Delta psi-driven transport of NH4+ through this protein. Expression of AtAMT1;1 in a novel yeast mutant defective in endogenous ammonium transport and vacuolar acidification supported the above mechanism for AtAMT1;1 and revealed a central role for acid vacuoles in storage and retention of ammonia in cells. These results highlight the mechanistic differences between plant AMT proteins and related transporters in bacteria and animal cells, and suggest novel strategies to enhance nitrogen use efficiency in agriculture. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Título según WOS: ID WOS:000238964800027 Not found in local WOS DB
Título de la Revista: FEBS LETTERS
Volumen: 580
Número: 16
Editorial: Wiley
Fecha de publicación: 2006
Página de inicio: 3931
Página final: 3936


Notas: ISI