DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS

Mundaca-Uribe, Rodolfo; De Diego, Marta; Henriquez-Aedo, Karem; Aranda, Mario.; Pena-Farfal, Carlos

Abstract

Acetazolamide (ACZ) is a carbonic anhydrase inhibitor that exhibits diuretic activity. In medicine, it is principally used in open-angle glaucoma. for prevention or amelioration of symptoms associated with acute high-altitude sickness and as an adjunct to other anticonvulsants in centrencephalic epilepsies. Furthermore, ACZ is sometimes used by athletes to mask the presence of doping substances, so its use has been banned in competitions. The aim of this work was the development of a sensor to determine ACZ, in pharmaceuticals. as quality control way, and serum, for clinical and doping analyzes. The sensor has been developed modifying a glassy carbon electrode with carbon nanofibers. The electrochemical characterization of the sensor was performed by cyclic voltammetry using the redox mediator potassium ferrocyanide. ACZ electiuchemical behavior was studied as well. Measurements were performed in a conventional three electrodes cell using differential pulse voltammetry. The influence of some experimental variables involved in the preparation and performance of the sensor -amount of modifier, supporting electrolyte, pH, scan rate and pulse amplitude- were optimized. This methodology provided a linear calibration plot for ACZ in the 1 - 17 AM concentration range. Furthermore, the proposed sensor exhibited suitable analytical properties, with detection and quantification limits of 0.06 mu M and 0.2 mu M respectively; analytical sensitivity of 1.142 mu A mu M, repeatability of 0.72 %, and it was applied to determine ACZ, in pharmaceuticals (tablets) and spiked human serum, being an excellent tool to perform quality control and antidoping control.

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Título según WOS: DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
Título según SCOPUS: Development and characterization of a sensor based on carbon nanofibers: Application to acetazolamide determination in pharmaceuticals and biological fluids
Título según SCIELO: DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
Título de la Revista: Journal of the Chilean Chemical Society
Volumen: 64
Número: 1
Editorial: Sociedad Chilena de Química
Fecha de publicación: 2019
Página de inicio: 4382
Página final: 4385
Idioma: English
DOI:

10.4067/s0717-97072019000104382

Notas: ISI, SCIELO, SCOPUS