Characterization of the petI and res operons of Acidithiobacillus ferrooxidans

Levicán G; Bruscella, P; Guacunano, M; Inostroza C.; Bonnefoy V.; Holmes, DS; Jedlicki E

Abstract

DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-PCR experiments suggest that the two clusters are organized as operons, and +1 start sites of transcription for the operons have been determined by primer extension experiments. Potential promoters have been identified. The presence of these operons lends support to a recent model of reverse electron flow and is consistent with previous reports of phenotypic switching in this bacterium.

Más información

Título según WOS: Characterization of the petI and res operons of Acidithiobacillus ferrooxidans
Título según SCOPUS: Characterization of the petI and res operons of Acidithiobacillus ferrooxidans
Título de la Revista: JOURNAL OF BACTERIOLOGY
Volumen: 184
Número: 5
Editorial: AMER SOC MICROBIOLOGY
Fecha de publicación: 2002
Página de inicio: 1498
Página final: 1501
Idioma: English
URL: http://jb.asm.org/cgi/doi/10.1128/JB.184.5.1498-1501.2002
DOI:

10.1128/JB.184.5.1498-1501.2002

Notas: ISI, SCOPUS