Nuclear Sm antigens in the sperm of different organisms

Delgado, Fernando; Brito, Monica; Concha, Ilona; Schroeder, Ricardo; Burzio, Luis


Immunoblot analysis of sperm protein from several species revealed the presence of polypeptides recognised by anti-Sm sera obtained from patients with systemic lupus erythematosus. Immunoreactive polypeptides in human, bull, mouse and rat sperm were identified as protein B', B and D as compared with the Sm polypeptides of HeLa cells. In the sperm of rooster, the teleost fish Cyprinus carpio and the mussel Choromytilus chorus, the immunoreactive polypeptide profile was more complex. To ascertain the sperm origin of the Sm antigens, immunolocalisation with anti-Sm serum was carried out. The results demonstrated that in all the species studied staining was confined to the sperm nucleus, confirming that some polypeptides of the small nuclear ribonucleoprotein complex are present in the gamete.

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Título de la Revista: ZYGOTE
Volumen: 2
Número: 3
Fecha de publicación: 1994
Página de inicio: 227
Página final: 235
Idioma: english
Notas: ISI