Non-selective cation channels and oxidative stress- induced cell swelling

Simon F.; Varela D.; Riveros A.; Eguiguren, AL; Stutzin A.

Abstract

"Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (Barros et al., 2001a). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for activation and is inhibited by flufenamic acid. Hydrogen peroxide induced a significant increase in cell volume that was dependent on external Na+. We propose that the NSCC, which is ubiquitous though largely inactive in healthy cells, becomes activated under severe oxidative stress. The ensuing Na+ influx initiates via positive feedback a series of metabolic and electrolytic disturbances, resulting in cell death by necrosis"

Más información

Título según WOS: Non-selective cation channels and oxidative stress-induced cell swelling
Título según SCOPUS: Non-selective cation channels and oxidative stress-induced cell swelling
Título según SCIELO: Non-selective cation channels and oxidative stress- induced cell swelling
Título de la Revista: Biological Research
Volumen: 35
Número: 2
Editorial: Sociedad de Biología de Chile
Fecha de publicación: 2002
Página de inicio: 215
Página final: 222
Idioma: English
DOI:

10.4067/S0716-97602002000200013

Notas: ISI, SCIELO, SCOPUS