Technique validation by liquid chromatography for the determination of acyclovir in plasma

Fernandez, M. ; Sepulveda, J; Aranguiz, T; Von Plessing, C

Abstract

In this research project, a high-performance liquid chromatography (HPLC) method was developed for the determination of acyclovir (ACV) in plasma. The plasma samples, recharged with acyclovir and in presence of 5?-N-methylcarboxyamidoadenosine (MECA) as an internal standard, were purified using a solid-phase extraction technique with Waters Oasis HLB columns. The separation of the components from the extract was carried out in a LiChrospher 100 RP-18 column for further ultraviolet detection at a wavelength range of 250-260 nm. The mobile phase composition was 18% acetonitrile, sodium dodecylsulphate 5 mM and phosphate buffer at pH 2.6 with an analysis time of 13 min per sample. The average retention time for acyclovir was of 5.0 min and for the internal standard 11.2 min. The calibration curve was linear ranging between 0.05 and 1.80 ?g/ml. The detection limit was 0.006 ?g/ml with a quantification limit of 0.020 ?g/ml. The ACV recuperation percentage for 250 ?l of plasma was between 94.7 and 109.7% with a coefficient of variation not higher than 5.2%. This method was developed and validated for use in bioavailability and bioequivalence studies. © 2003 Elsevier Science B.V. All rights reserved.

Más información

Título según WOS: Technique validation by liquid chromatography for the determination of acyclovir in plasma
Título según SCOPUS: Technique validation by liquid chromatography for the determination of acyclovir in plasma
Título de la Revista: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volumen: 791
Número: 01-feb
Editorial: Elsevier
Fecha de publicación: 2003
Página de inicio: 357
Página final: 363
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S1570023203002526
DOI:

10.1016/S1570-0232(03)00252-6

Notas: ISI, SCOPUS