Protective effect of inactivated blastoconidia in keratinocytes and human reconstituted epithelium against C. albicans infection

Alburquenque C.; Amaro J.; Fuentes M.; Falconer M.A.; Moreno C.; Covarrubias C.; Pinto C.; Rodas P.I.; Bucarey S.A.; Hermosilla G.; Magne F.; Tapia C.V.

Abstract

Candida albicans is commensal yeast that colonizes skin and mucosa; however, it can become an opportunist pathogen by changing from blastoconidia (commensal form) into hypha (pathogenic form). Each form activates a different cytokines response in epithelial cells. Little is known about the commensal role of C. albicans in the innate immunity. This work studied whether stimulation with C. albicans blastoconidia induces protection in keratinocytes and/or in a reconstituted human epithelium (RHE) infected with C. albicans. For this, inactivated C. albicans blastoconidia was used to stimulate keratinocytes and RHE prior to infection with C. albicans. Blastoconidia induced different cytokine expression profiles; in the case of RHE it decreased interleukin (IL)-1 beta and IL-10 and increased IL-8, tumor necrosis factor a (TNF-alpha), and interferon. (IFN-alpha). A significant increase in the expression of human beta-defensins (HBD) 2 and HBD3 was observed in blastoconidia stimulated keratinocytes and RHE, associated with impaired growth and viability of C. albicans. Additionally, blastoconidia stimulation decreased the expression of virulence factors in C. albicans that are associated with filamentation (EFG1, CPH1 and NRG1), adhesion (ALS5), and invasion (SAP2). Blastoconidia stimulated RHE was significantly less damaged by C. albicans invasion. These results show that the commensal form of C. albicans would exert a protective effect against self-infection.

Más información

Título según WOS: Protective effect of inactivated blastoconidia in keratinocytes and human reconstituted epithelium against C. albicans infection
Título según SCOPUS: Protective effect of inactivated blastoconidia in keratinocytes and human reconstituted epithelium against C. Albicans infection
Título de la Revista: MEDICAL MYCOLOGY
Volumen: 57
Número: 4
Editorial: Oxford University Press
Fecha de publicación: 2019
Página de inicio: 457
Página final: 467
Idioma: English
DOI:

10.1093/mmy/myy068

Notas: ISI, SCOPUS