Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus

Villanueva, RA; Galaz, JL; Valdes, JA; Jashes, MM; Sandino, AM

Abstract

In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.

Más información

Título según WOS: Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus
Título según SCOPUS: Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus
Título de la Revista: JOURNAL OF VIROLOGY
Volumen: 78
Número: 24
Editorial: AMER SOC MICROBIOLOGY
Fecha de publicación: 2004
Página de inicio: 13829
Página final: 13838
Idioma: English
URL: http://jvi.asm.org/cgi/doi/10.1128/JVI.78.24.13829-13838.2004
DOI:

10.1128/JVI.78.24.13829-13838.2004

Notas: ISI, SCOPUS