Effects of phosphorylation by protein kinase CK2 on the human basal components of the RNA polymerase II transcription machinery

Cabrejos, ME; Allende, CC; Maldonado E.

Abstract

We have investigated the role of phosphorylation by vertebrate protein kinase CK2 on the activity of the General Transcription Factors TFIIA, TFIIE, TFIIF, and RNAPII. The largest subunits of TFIIA, TFIIE, and TFIIF were phosphorylated by CK2 holoenzyme. Also, RNA polymerase II was phosphorylated by CK2 in the 214,000 and 20,500 daltons subunits. Our results show that phosphorylation of TFIIA, TFIIF, and RNAPII increase the formation of complexes on the TATA box of the Ad-MLP promoter. Also, phosphorylation of TFIIF increases the formation of transcripts, where as phosphorylation of RNA polymerase II dramatically inhibits transcript formation. Furthermore, we demonstrate that CK2? directly interacts with RNA polymerase II, TFIIA, TFIIF, and TBP. These results strongly suggest that CK2 may play a role in regulating transcription of protein coding genes. © 2004 Wiley-Liss, Inc.

Más información

Título según WOS: Effects of phosphorylation by protein kinase CK2 on the human basal components of the RNA polymerase II transcription machinery
Título según SCOPUS: Effects of phosphorylation by protein kinase CK2 on the human basal components of the RNA polymerase II transcription machinery
Título de la Revista: JOURNAL OF CELLULAR BIOCHEMISTRY
Volumen: 93
Número: 1
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2004
Página de inicio: 2
Página final: 10
Idioma: English
URL: http://doi.wiley.com/10.1002/jcb.20209
DOI:

10.1002/jcb.20209

Notas: ISI, SCOPUS