Arabidopsis Heterotrimeric G-protein Regulates Cell Wall Defense and Resistance to Necrotrophic Fungi

Delgado-Cerezo, Magdalena; Sanchez-Rodriguez, Clara; Escudero, Viviana; Miedes, Eva; Virginia Fernandez, Paula; Jorda, Lucia; Hernandez-Blanco, Camilo; Sanchez-Vallet, Andrea; Bednarek, Pawel; Schulze-Lefert, Paul; Somerville, Shauna; Manuel Estevez, Jose; Persson, Staffan; Molina, Antonio


The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the G beta subunit displays enhanced susceptibility to these pathogens. G beta/AGB1 forms an obligate dimer with either one of the Arabidopsis G gamma subunits (gamma 1/AGG1 and gamma 2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the G beta and G gamma 1/gamma 2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.

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Título según WOS: ID WOS:000299347400008 Not found in local WOS DB
Título de la Revista: MOLECULAR PLANT
Volumen: 5
Número: 1
Editorial: Cell Press
Fecha de publicación: 2012
Página de inicio: 98
Página final: 114


Notas: ISI