Usage of supercritical fluid techniques to obtain bioactive alkaloid-rich extracts from cherimoya peel and leaves: extract profiles and their correlation with antioxidant properties and acetylcholinesterase and alpha-glucosidase inhibitory activities

Galarce-Bustos, Oscar; Teresa Fernandez-Ponce, Ma; Montes, Antonio; Pereyra, Clara; Casas, Lourdes; Mantell, Casimiro; Aranda, Mario.

Abstract

The agroindustrial sector is highly concerned with regards to reducing the environmental impact of waste from pruning activities (leaves, branches and bark) and from food industry processes (peels and seeds). In this sense, the wastes generated by cherimoya cultivation and processing industries should be contemplated as a valuable source of biologically active compounds. In this work, we have studied the bioactivity of alkaloid-rich Annona cherimola Mill. extracts obtain by means of supercritical fluid extraction techniques. The extracts were obtained from the peel and leaves using the following optimal conditions: 100 bar of pressure, 75 degrees C and 15% methanol as co-solvent. High antioxidant capacity (5304.23 +/- 73.60 to 21 705.20 +/- 1069.31 mu mol Trolox equivalent per 100 g), and acetylcholinesterase (IC50 = 87.69 +/- 3.42 to 515.02 +/- 29.25 mu g mL(-1)) and alpha-glucosidase (IC50 = 1097.76 +/- 121.12 to 3206.88 +/- 97.06 mu g mL(-1)) inhibitory activities were exhibited by both peel and leaf extracts. Larger alkaloid contents were determined by UHPLC-ESI-MS analysis, with peel extracts presenting a high concentration of N-trans-feruloyl phenethylamine, while leaf extracts were rich in anonine. This work reports novel data on bioactivity of cherimoya peel and leaves and their potential as a source of bioactive compounds.

Más información

Título según WOS: Usage of supercritical fluid techniques to obtain bioactive alkaloid-rich extracts from cherimoya peel and leaves: extract profiles and their correlation with antioxidant properties and acetylcholinesterase and alpha-glucosidase inhibitory activities
Título de la Revista: FOOD & FUNCTION
Volumen: 11
Número: 5
Editorial: ROYAL SOC CHEMISTRY
Fecha de publicación: 2020
Página de inicio: 4224
Página final: 4235
DOI:

10.1039/D0FO00342E

Notas: ISI