METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

Gómez C; Von Plessing, C; Godoy, CG; Reinbach, R; Godoy, R

Abstract

A practical reversed-phase high-performance liquid chromatography (HPLC) method was developed for 4-chloro-N-furfuryl-5-sulphamoylanthranilic acid (furosemide) determination in plasma. The plasma samples spiked with the drug in presence of propranolol hydrochloride as internal standard, were purified using a liquid - liquid extraction technique. The drugs were separated through a Kromasil 100-5C18 column with a mobile phase composed of acetonitrile and 0.02 M potassium dihydrogen phosphate (34 : 66 v/v) adjusted to pH 3.0 and quantified by fluorescence detection (excitation at 268 nm, emission at 410 nm). The calibration curve was linear ranging between 0.005 and 1.500 mg/mL. The detection limit was 0.001mg/mL and the quantification limit 0.003 mg/mL in plasma. The furosemide recovery from 1mL of plasma was between 99.8 and 101.6 %, with a variation coefficient not higher than 4,85 %. The method is sensitive enough for use in bioavailability and bioequivalence studies after oral administration of 40 mg (a tablet) of furosemide

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Título según WOS: Method validation for the determination of furosemide in plasma by liquid-liquid extraction and high-performance liquid chromatography with fluorescence detection
Título según SCIELO: METHOD VALIDATION FOR THE DETERMINATION OF FUROSEMIDE IN PLASMA BY LIQUID- LIQUID EXTRACTION AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
Título de la Revista: Journal of the Chilean Chemical Society
Volumen: 50
Número: 2
Editorial: Sociedad Chilena de Química
Fecha de publicación: 2005
Página de inicio: 479
Página final: 482
Idioma: English
DOI:

10.4067/S0717-97072005000200008

Notas: ISI, SCIELO