A Circadian Clock in Neurospora Crassa Functions During Plant Cell Wall Deconstruction

Díaz, Rodrigo D.; Larrondo, Luis F.

Keywords: circadian, cellulose, Neurospora


Circadian clocks are autonomous timers that are believed to confer organisms a selective advantage by enabling processes to occur at appropriate times of the day. In the model fungus Neurospora crassa, 20-40 % of its genes are reported to be under circadian regulation, as assayed in simple sugar media. Although it has been well-described that Neurospora efficiently deconstructs plant cell wall components, little is known regarding the status of the clock when Neurospora grows on cellulosic material, or whether such a clock has an impact on any of the genes involved in this process. Through luciferase-based reporters and fluorescent detection assays, we show that a clock is functioning when Neurospora grows on cellulose-containing wheat straw as the only carbon and nitrogen source. Additionally, we found that the major cellobiohydrolase encoding gene involved in plant cell wall deconstruction, cbh-1, is rhythmically regulated by the Neurospora clock, in a manner that depends on cellulose concentration and on the transcription factor CRE-1, known as a key player in carbon-catabolite repression in this fungus. Our findings are a step towards a more comprehensive understanding on how clock regulation modulates cellulose degradation, and thus Neurospora's physiology. PMID: 32389313

Más información

Título de la Revista: FUNGAL BIOLOGY
Volumen: 124
Número: 5
Fecha de publicación: 2020
Página de inicio: 501
Página final: 508
Idioma: English
URL: 10.1016/j.funbio.2020.03.003