Deficient expression of monoamine oxidase A in the endometrium is associated with implantation failure in women participating as recipients in oocyte donation

Henríquez S.; Tapia, A; Quezada M.; Vargas, M.; Cardenas H.; Rios M.; Salvatierra AM; Croxatto H.; Orihuela, P; Zegers-Hochschild, F; Munroe, DJ; Velasquez L.

Abstract

Successful implantation depends both on the quality of the embryo and on the endometrial receptivity. The latter depends on progesterone-induced changes in gene expression, a process that has been characterized by microarray analysis. One of the genes whose transcription appears to be enhanced during the receptive period is monoamine oxidase A (MAO-A). Our first objective was to confirm the increased expression of MAO-A in the endometrium during the receptive phase of spontaneous normal cycles using real time PCR and immunofluorescence. The second objective was to examine the endometrial expression of MAO-A during the receptive phase induced by exogenous estradiol (E2) and progesterone in patients whose endometrium was shown to have been either receptive or non-receptive to embryo implantation in repeated cycles of oocyte donation. Results showed that MAO-A transcript levels increased between the pre-receptive (LH+3) and receptive phase (LH+7) in all spontaneous cycles examined, with a median increase of 25-fold. Immunofluorescent labelling demonstrated MAO-A localization to the glandular and luminal epithelium with an increasing positive score between LH+3 and LH+7. Conversely, prior failure of embryo implantation was associated with a 29-fold decrease in MAO-A mRNA levels and a substantial reduction in MAO-A protein immunofluorescent label score. These results show a strong association between endometrial receptivity and MAO-A expression in the endometrial epithelium, suggesting an important role for this enzyme in normal implantation. © 2006 Oxford University Press.

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Título según WOS: Deficient expression of monoamine oxidase A in the endometrium is associated with implantation failure in women participating as recipients in oocyte donation
Título según SCOPUS: Deficient expression of monoamine oxidase A in the endometrium is associated with implantation failure in women participating as recipients in oocyte donation
Título de la Revista: MOLECULAR HUMAN REPRODUCTION
Volumen: 12
Número: 12
Editorial: OXFORD UNIV PRESS
Fecha de publicación: 2006
Página de inicio: 749
Página final: 754
Idioma: English
URL: http://www.molehr.oxfordjournals.org/cgi/doi/10.1093/molehr/gal082
DOI:

10.1093/molehr/gal082

Notas: ISI, SCOPUS