High Fat Diet-induced Skeletal Muscle Wasting Is Decreased by Mesenchymal Stem Cells Administration: Implications on Oxidative Stress, Ubiquitin Proteosome Pathway Activation, and Myonuclear Apoptosis
Keywords: oxidative stress, stem cells, skeletal muscle atrophy, Skeletal muscle wasting, High Fat-Diet, Ubiquitin proteosome, Myonuclear apoptosis
Obesity can lead to skeletal muscle atrophy, a pathological condition characterized by the loss of strength and muscle mass. A feature of muscle atrophy is a decrease of myofibrillar proteins as a result of ubiquitin proteasome pathway overactivation, as evidenced by increased expression of the muscle-specific ubiquitin ligases atrogin-1 and MuRF-1. Additionally, other mechanisms are related to muscle wasting, including oxidative stress, myonuclear apoptosis, and autophagy. Stem cells are an emerging therapy in the treatment of chronic diseases such as high fat diet-induced obesity. Mesenchymal stem cells (MSCs) are a population of self-renewable and undifferentiated cells present in the bone marrow and other mesenchymal tissues of adult individuals. The present study is the first to analyze the effects of systemic MSC administration on high fat diet-induced skeletal muscle atrophy in the tibialis anterior of mice. Treatment with MSCs reduced losses of muscle strength and mass, decreases of fiber diameter and myosin heavy chain protein levels, and fiber type transitions. Underlying these antiatrophic effects, MSC administration also decreased ubiquitin proteasome pathway activation, oxidative stress, and myonuclear apoptosis. These results are the first to indicate that systemically administered MSCs could prevent muscle wasting associated with high fat diet-induced obesity and diabetes.
|Título de la Revista:||OXIDATIVE MEDICINE AND CELLULAR LONGEVITY|
|Fecha de publicación:||2016|
|Página de inicio:||1|
|Financiamiento/Sponsor:||This study was supported by research grants from the Association-Française Contre Les Myopathies AFM 16670 (Claudio Cabello-Verrugio); FONDECYT 1161646 (Claudio Cabello-Verrugio), 1150589 (Marcelo Ezquer), 1161288 (Felipe Simon), and 3140396 (Daniel Cabre|