Adenosine triphosphate, polymyxin B and B16 cell-derived immunization induce anticancer response

Barrera-Avalos, Carlos; Mena, Javier; Lopez, Ximena; Cappelli, Claudio; Neira, Tanya; Imarai, Monica; Fernandez, Ricardo; Robles-Planells, Claudia; Rojo, Leonel E.; Milla, Luis A.; Leiva-Salcedo, Elias; Escobar, Alejandro; Acuna-Castillo, Claudio

Abstract

Aim: Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. Materials methods: We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the in vivo antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). Results: The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8(+) lymphocytes in vitro. Conclusion: Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine.

Más información

Título según WOS: ID WOS:000604907900001 Not found in local WOS DB
Título de la Revista: IMMUNOTHERAPY
Volumen: 13
Número: 4
Editorial: Future Medicine Ltd.
Fecha de publicación: 2021
Página de inicio: 309
Página final: 326
DOI:

10.2217/imt-2020-0209

Notas: ISI