Evaluation of BOX-PCR and REP-PCR as Molecular Typing Tools for AntarcticStreptomyces

Borba, Marcela Proenca; Ballarini, Ana Elisa; Witusk, Joao Paulo Duarte; Lavin, Paris; Van der Sand, Sueli


Molecular studies led to the resurgence of natural products research from genusStreptomyces, already known for their long history and importance for the pharmaceutical industry. However, species belonging to this genus are difficult to identify and the most commonly used techniques, which are based on 16S rRNA sequencing, do not discriminate between related species. In this work, amplification profiles generated from BOX-PCR and REP-PCR of 49 Antarctic soil streptomycetes were compared to evaluate the diversity present in the group and to characterize the bacterial isolates, along with some 16S rRNA amplifications. The BOX-A1R primer exhibit clearer amplification fragments, different from the amplification patterns obtained using the REP 1R and 2R primers. A higher diversity was observed with REP-PCR amplifications, even though a larger number of fragments was obtained with BOX-A1R primer amplifications. There are at least four isolates that showed great similarity (about 90%) in both techniques. In other hand, there are two others that are 90% similar in BOX-PCR, but distant in REP-PCR, showing only 40% of similarity. Results of the combination of BOX-PCR and REP-PCR represent a simple and low-cost method to discriminate betweenStreptomycesstrains. There is no species identification with only the 16S rRNA, most isolates seem to be related toS. globisporus. Further studies added to the obtained results may provide better data to help the characterization of these microorganisms.

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Título según WOS: Evaluation of BOX-PCR and REP-PCR as Molecular Typing Tools for AntarcticStreptomyces
Título de la Revista: CURRENT MICROBIOLOGY
Volumen: 77
Número: 11
Editorial: Springer
Fecha de publicación: 2020
Página de inicio: 3573
Página final: 3581


Notas: ISI