Molecular characterization of Trichinella genotypes by inter-simple sequence repeat polymerase chain reaction (ISSR-PCR)

Fonseca-Salamanca, F; Nogal-Ruiz, JJ; Benito C.; Camacho, MV; Martinez-Fernandez, AR

Abstract

A bulk analysis of inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) provides a quick, reliable, and highly informative system for DNA banding patterns that permit species identification. The present study evaluates the applicability of this system to Trichinella species identification. After a single amplification carried out on a single larva with the primer 816([CA]nRY) under high stringency conditions, which provide high reproducibility, we were able to identify by consistent banding patterns 5 sibling species: Trichinella spiralis (ISS48), 2 Trichinella britovi isolates (ISSII and ISS86), Trichinella murrelli (ISS35), Trichinella nativa (ISS71), Trichinella nelsoni (ISS29); 3 additional Trichinella genotypes: T8 (ISS149), T9 (ISS408 and ISS409), and T6 (ISS34); and the nonencapsulated species Trichinella pseudospiralis (ISS13). Moreover, 33 new Trichinella isolates from 2 zoogeographical regions were unequivocally identified. All Trichinella isolates have shown an identical pattern with those produced by the reference strain. According to these data, we have demonstrated that ISSR-PCR is a robust technique that emerges as a useful new application for the molecular identification of Trichinella isolates in epidemiological studies. © American Society of Parasitologists 2006.

Más información

Título según WOS: Molecular characterization of Trichinella genotypes by inter-simple sequence repeat polymerase chain reaction (ISSR-PCR)
Título según SCOPUS: Molecular characterization of Trichinella genotypes by inter-simple sequence repeat polymerase chain reaction (ISSR-PCR)
Título de la Revista: JOURNAL OF PARASITOLOGY
Volumen: 92
Número: 3
Editorial: AMER SOC PARASITOLOGISTS
Fecha de publicación: 2006
Página de inicio: 606
Página final: 610
Idioma: English
URL: http://www.bioone.org/doi/abs/10.1645/GE-678R.1
DOI:

10.1645/GE-678R.1

Notas: ISI, SCOPUS