Suitcase Lab: new, portable, and deployable equipment for rapid detection of specific harmful algae in Chilean coastal waters

Fujiyoshi, So; Yarimizu, Kyoko; Miyashita, Yohei; Rilling, Joaquin; Acuna, Jacquelinne J.; Ueki, Shoko; Gajardo, Gonzalo; Espinoza-Gonzalez, Oscar; Guzman, Leonardo; Jorquera, Milko A.; Nagai, Satoshi; Maruyama, Fumito

Abstract

Phytoplankton blooms, including harmful algal blooms (HABs), have serious impacts on ecosystems, public health, and productivity activities. Rapid detection and monitoring of marine microalgae are important in predicting and managing HABs. We developed a toolkit, the Suitcase Lab, to detect harmful algae species in the field. We demonstrated the Suitcase Lab's capabilities for sampling, filtration, DNA extraction, and loop-mediated isothermal amplification (LAMP) detection in cultured Alexandrium catenella cells as well as Chilean coastal waters from four sites: Repollal, Isla Garcia, Puerto Montt, and Metri. A LAMP assay using the Suitcase Lab in the field confirmed microscopic observations of A. catenella in samples from Repollal and Isla Garcia. The Suitcase Lab allowed the rapid detection of A. catenella, within 2 h from the time of sampling, even at a single cell per milliliter concentrations, demonstrating its usefulness for quick and qualitative on-site diagnosis of target toxic algae species. This method is applicable not only to detecting harmful algae but also to other field studies that seek a rapid molecular diagnostic test.

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Título según WOS: ID WOS:000590549300008 Not found in local WOS DB
Título de la Revista: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Editorial: SPRINGER HEIDELBERG
Fecha de publicación: 2020
DOI:

10.1007/s11356-020-11567-5

Notas: ISI