Puroindoline-a and alpha 1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells
Puroindoline-a (PIN-a) and Î±1-purothionin (Î±1-PTH), isolated from wheat endosperm of Triticum aestivum sp., have been suggested to play a role in plant defence mechanisms against phytopathogenic organisms. We investigated their ability to form pores when incorporated into giant liposomes using the patch-clamp technique. PIN-a formed cationic channels (â‰ˆ 15 pS) with the following selectivity K+ > Na+ â‰« Cl -. Also, Î±1-PTH formed channels of â‰ˆ 46 pS and 125 pS at +100 mV, the selectivity of which was Ca2+ > Na+ â‰ˆ K+ â‰« Cl- and Cl- â‰« Na+, respectively. In isolated mouse neuromuscular preparations, Î±1-PTH induced muscle membrane depolarization, leading to blockade of synaptic transmission and directly elicited muscle twitches. Also, Î±1-PTH caused swelling of differentiated neuroblastoma NG108-15 cells, membrane bleb formation, and disorganization of F-actin. In contrast, similar concentrations of PIN-a had no detectable effects. The cytotoxic actions of Î±1-PTH on mammalian cells may be explained by its ability to induce cationic-selective channels. Â© 2006 The Authors.
|Título según WOS:||Puroindoline-a and alpha 1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells|
|Título según SCOPUS:||Puroindoline-a and a1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells|
|Título de la Revista:||FEBS JOURNAL|
|Fecha de publicación:||2006|
|Página de inicio:||1710|