Lymphoid B cells induce NF-kappa B activation in high endothelial cells from human tonsils
Immune surveillance depends on still poorly understood lymphocyte-endothelium interactions required for lymphocyte transendothelial migration into secondary lymphoid organs. The nuclear factor ÎºB (NF-ÎºB) regulatory system and its inhibitory IÎºB proteins control the inducible expression of adhesion molecules, cytokines and chemokines involved in endothelial activation and lymphocyte transmigration. Here we present results showing the activation of this system in response to the interaction of high endothelial cells from human tonsils (HUTEC) with human B and T lymphoid cell lines and primary tonsillar lymphocytes. Western blot and electrophoretic mobility shift assays show that adhesion of different lymphoid cells induce varying levels of NF-ÎºB activation in HUTEC, with Daudi cells, tonsil-derived B cell line 10 (TBCL-10) and primary tonsillar B lymphocytes causing the strongest activation. The main NF-ÎºB protein complexes translocated to the nucleus were p65/p50 and p50/p50. Results from reverse transcription-PCR and flow cytometry analysis of HUTEC indicate that the interaction with Daudi cells induce an increased expression of IL-6 and IL-8 mRNA and cell-surface expression of intercellular adhesion molecule-1, all of which were prevented by sodium salicylate, an inhibitor of NF-ÎºB activation. Transwell experiments show that NF-ÎºB activation and the response of HUTEC to the interaction of Daudi cells does not depend on direct cell-cell contact but rather on the production of soluble factors that require the presence of both cell types. These results suggest that lymphocytes and high endothelium establish a cross talk leading to NF-ÎºB-mediated expression of cytokines and adhesion molecules, inducing endothelial cell activation. Â© The Japanese Society for Immunology. 2005. All rights reserved.
|Título según WOS:||Lymphoid B cells induce NF-kappa B activation in high endothelial cells from human tonsils|
|Título según SCOPUS:||Lymphoid B cells induce NF-?B activation in high endothelial cells from human tonsils|
|Título de la Revista:||INTERNATIONAL IMMUNOLOGY|
|Editorial:||OXFORD UNIV PRESS|
|Fecha de publicación:||2006|
|Página de inicio:||259|