Novel Pannexin-1-Coupled Signaling Cascade Involved in the Control of Endothelial Cell Function and NO-Dependent Relaxation

Lillo, Mauricio A.; Gaete, Pablo S.; Puebla, Mariela; Burboa, Pia C.; Poblete, Ines; Figueroa, Xavier F.


Deletion of pannexin-1 (Panx-1) leads not only to a reduction in endothelium-derived hyperpolarization but also to an increase in NO-mediated vasodilation. Therefore, we evaluated the participation of Panx-1-formed channels in the control of membrane potential and [Ca2+](i) of endothelial cells. Changes in NO-mediated vasodilation, membrane potential, superoxide anion (O-2(-)) formation, and endothelial cell [Ca2+](i) were analyzed in rat isolated mesenteric arterial beds and primary cultures of mesenteric endothelial cells. Inhibition of Panx-1 channels with probenecid (1 mM) or the Panx-1 blocking peptide (10)Panx (60 mu M) evoked an increase in the ACh (100 nM)-induced vasodilation of KCl-contracted mesenteries and in the phosphorylation level of endothelial NO synthase (eNOS) at serine 1177 (P-eNOS(S1177)) and Akt at serine 473 (P-Akt(S473)). In addition, probenecid or (10)Panx application activated a rapid, tetrodotoxin (TTX, 300 nM)-sensitive, membrane potential depolarization and [Ca2+](i) increase in endothelial cells. Interestingly, the endothelial cell depolarization was converted into a transient spike after removing Ca2+ ions from the buffer solution and in the presence of 100 mu M mibefradil or 10 mu M Ni2+. As expected, Ni2+ also abolished the increment in [Ca2+](i). Expression of Na(v)1.2, Na(v)1.6, and Ca(v)3.2 isoforms of voltage-dependent Na+ and Ca2+ channels was confirmed by immunocytochemistry. Furthermore, the Panx-1 channel blockade was associated with an increase in O-2(-) production. Treatment with 10 mu M TEMPOL or 100 mu M apocynin prevented the increase in O-2(-) formation, ACh-induced vasodilation, P-eNOS(S1177), and P-Akt(S473) observed in response to Panx-1 inhibition. These findings indicate that the Panx-1 channel blockade triggers a novel complex signaling pathway initiated by the sequential activation of TTX-sensitive Na-v channels and Ca(v)3.2 channels, leading to an increase in NO-mediated vasodilation through a NADPH oxidase-dependent P-eNOS(S1177), which suggests that Panx-1 may be involved in the endothelium-dependent control of arterial blood pressure.

Más información

Título según WOS: Novel Pannexin-1-Coupled Signaling Cascade Involved in the Control of Endothelial Cell Function and NO-Dependent Relaxation
Título de la Revista: Oxidative Medicine and Cellular Longevity
Volumen: 2021
Editorial: HINDAWI LTD
Fecha de publicación: 2021


Notas: ISI