Recapitulation of prostate tissue cell type-specific transcriptomes by anin vivoprimary prostate tissue xenograft model

Gross, Nelson T.; Wang, Jianmin; Fiandalo, Michael V.; Cortes Gomez, Eduardo; Watts, Anica; Godoy, Alejandro S.; Smith, Gary J.; Wu, Yue

Abstract

Studies of the normal functions and diseases of the prostate requestin vivomodels that maintain the tissue architecture and the multiple-cell type compartments of human origin in order to recapitulate reliably the interactions of different cell types. Cell type-specific transcriptomes are critical to reveal the roles of each cell type in the functions and diseases of the prostate. A primary prostate tissue xenograft model was developed using fresh human prostate tissue specimens transplanted onto male mice that were castrated surgically and implanted with a device to maintain circulating testosterone levels comparable to adult human males. Endothelial cells and epithelial cells were isolated from 7 fresh human prostate tissue specimens and from primary tissue xenografts established from 9 fresh human prostate tissue specimens, using antibody-conjugated magnetic beads specific to human CD31 and human EpCAM, respectively. Transcriptomes of endothelial, epithelial and stromal cell fractions were obtained using RNA-Seq. Global and function-specific gene expression profiles were compared in inter-cell type and inter-tissue type manners. Gene expression profiles in the individual cell types isolated from xenografts were similar to those of cells isolated from fresh tissue, demonstrating the value of the primary tissue xenograft model for studies of the inter-relationships between prostatic cell types and the role of such inter-relationships in organ development, disease progression, and response to drug treatments.

Más información

Título según WOS: ID WOS:000545747200093 Not found in local WOS DB
Título de la Revista: PLOS ONE
Volumen: 15
Número: 6
Editorial: Public Library of Science
Fecha de publicación: 2020
DOI:

10.1371/journal.pone.0233899

Notas: ISI