Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

Swearingen, JW; Fuentes DE; Araya, MA; Plishker, MF; Saavedra, CP; Chasteen, TG; Vásquez CC

Abstract

The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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Título según WOS: Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures
Título según SCOPUS: Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures
Título de la Revista: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volumen: 72
Número: 1
Editorial: American Society for Microbiology
Fecha de publicación: 2006
Página de inicio: 963
Página final: 967
Idioma: English
URL: http://aem.asm.org/cgi/doi/10.1128/AEM.72.1.963-967.2006
DOI:

10.1128/AEM.72.1.963-967.2006

Notas: ISI, SCOPUS