TUBULIN-TYROSINE LIGASE CATALYZES COVALENT BINDING OF 3-FLUORO-TYROSINE TO TUBULIN - KINETIC AND [F-19]NMR STUDIES
The use of 3-fluoro-tyrosine as an alternative substrate for the enzyme tubulin:tyrosine ligase which catalyzes the incorporation of tyrosine into the alpha-tubulin subunit was investigated, The incorporation of tyrosine into tubulin was inhibited competitively by 3-fluoro-tyrosine with an apparent K-i of similar to 25 mu M. The affinity for this analog was similar to that of tyrosine, confirming that the hydrogen at position 3 of the aromatic ring is not essential for the reaction catalyzed by TTLase, The incorporation of 3-fluoro-tyrosine into the C-terminus of the alpha-tubulin subunit was demonstrated through [F-19]NMR spectroscopy, The 3-fluoro-tyrosine signal at -58.6 ppm (trifluoroacetic acid as external standard), with a bandwidth of 24.7 Hz presented a chemical shift of 0.75 ppm upheld and an enlargement in the bandwidth (30.5 Hz) when incorporated into tubulin, These results strongly suggest that this amino acid is exposed to the solvent in tubulin. Tubulin covalently labeled with 3-fluoro-tyrosine was competent to polymerize into microtubules. The use of fluorinated tubulin in [F-19]NMR spectroscopy for studying questions concerning protein conformation and interactions will be discussed.
|Título según WOS:||ID WOS:A1995TD12400005 Not found in local WOS DB|
|Título de la Revista:||FEBS LETTERS|
|Editorial:||ELSEVIER SCIENCE BV|
|Fecha de publicación:||1995|
|Página de inicio:||165|