Concentration of EPA and DHA from Refined Salmon oil by optimization the Urea. Fatty acid adduction reaction conditions using response Surface methodology

Dovale Rosabal, Gretel; Rodriguez, Alicia; Contreras, Elyzabeth; Ortiz-Viedma, Jaime; Muñoz, Marlys; Trigo Marcos; Aubourg, Santiago P.; Espinosa, Alejandra

Keywords: response surface methodology (rsm), dha, epa, desirability function, refined commercial salmon oil, n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFAs) concentration, EPA+DHA, total FA yield, process variable maximization, multiple response optimization


This research focused on obtaining eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) (EPA+DHA) concentrates from refined commercial salmon oil (RCSO). Independent variables of the complexation process were optimized by means of the application of response surface methodology (RSM) in order to obtain the maximum content of such fatty acids (FAs). As a result of employing the optimized conditions for all the variables (6.0, urea:FA content ratio; ????18.0 C, crystallization temperature; 14.80 h, crystallization time; 500 rpm, stirring speed), high contents of EPA and DHA could be obtained from RCSO, achieving increases of 4.1 and 7.9 times in the concentrate, with values of 31.20 and 49.31 g/100 g total FA, respectively. Furthermore, a 5.8-time increase was observed for the EPA + DHA content, which increased from 13.78 to 80.51 g/100 g total FA. It is concluded that RCSO can be transformed into a profitable source of EPA and DHA (EPA+DHA), thus leading to a product with higher commercial value

Más información

Título de la Revista: MOLECULES
Volumen: 24
Editorial: MDPI
Fecha de publicación: 2019
Página de inicio: 1642
Página final: 1654
Idioma: English