Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients

Soto, Yudira; Maria Limia, Celia; Gonzalez, Licet; Gra, Bienvenido; Marina Hano, Olga; Ariel Martinez, Pedro; Kouri, Vivian


The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.

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Título según WOS: ID WOS:000391437500002 Not found in local WOS DB
Volumen: 111
Número: 12
Editorial: Fundaco Oswaldo Cruz
Fecha de publicación: 2016
Página de inicio: 731
Página final: 736


Notas: ISI