Characterization of an Operon Encoding Two c-Type Cytochromes, an aa3-Type Cytochrome Oxidase, and Rusticyanin in Thiobacillus ferrooxidansATCC 33020

Appia-Ayme C., Guiliani N., Ratouchniak J.,Bonnefoy, V.

Keywords: Acidithiobacillus, Ferrous iron oxidation pathway

Abstract

Despite the importance of Thiobacillus ferrooxidans in bioremediation and bioleaching, little is known about the genes encoding electron transfer proteins implicated in its energetic metabolism. This paper reports the sequences of the fourcox genes encoding the subunits of anaa3-type cytochrome c oxidase. These genes are in a locus containing four other genes:cyc2, which encodes a high-molecular-weight cytochromec; cyc1, which encodes ac4-type cytochrome (c552); open reading frame 1, which encodes a putative periplasmic protein of unknown function; and rus, which encodes rusticyanin. The results of Northern and reverse transcription-PCR analyses indicated that these eight genes are cotranscribed. Two transcriptional start sites were identified for this operon. Upstream from each of the start sites was a ς70-type promoter recognized in Escherichia coli. While transcription in sulfur-grown T. ferrooxidans cells was detected from the two promoters, transcription in ferrous-iron-grown T. ferrooxidans cells was detected only from the downstream promoter. The cotranscription of seven genes encoding redox proteins suggests that all these proteins are involved in the same electron transfer chain; a model taking into account the biochemistry and the genetic data is discussed.

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Título de la Revista: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volumen: 65
Editorial: American Society for Microbiology
Fecha de publicación: 1999
Página de inicio: 4781
Página final: 4787
Idioma: english
URL: https://doi.org/10.1128/AEM.65.11.4781-4787.1999
Notas: WOS