The PCR-based detection of Trypanosoma cruzi in the faeces of Triatoma infestans fed on patients with chronic American trypanosomiasis gives higher sensitivity and a quicker result than routine xenodiagnosis

Zulantay I.; Apt, W; Gic, LC; Rocha C.; Mundaca, K; Solari, A; Sánchez G; Rodriguez, C; Martinez, G; De Pablost, LM; Sandoval L.; Rodriguez J.; Vilchez, S; Osuna A.

Abstract

In the xenodiagnosis (XD) of American trypanosomiasis (Chagas disease), Trypanosoma cruzi in the triatomine bugs fed on the patient can now be detected using PCR (XD-PCR) as well as by microscopy (XD-M). In a study to compare XD-PCR with XD-M, triatomine bugs were fed on 50 cases of chronic American trypanosomiasis, of whom only 25 were ever found positive by XD-M. Overall, the bugs fed on 34 of the patients (all 25 cases found positive by XD-M and nine of the other patients) were found PCR-positive, giving a 330-bp fragment corresponding to part of the hypervariable region of the kinetoplast DNA of T. cruzi. Of the 25 patients who were ever found positive by XD-M, 20 gave bugs that were smear-positive on day 90 and a similar number (24; P=0.125) gave bugs that were PCR-positive at this time. On day 30, however, the bugs fed on only 11 of these 25 patients were found positive by microscopy, whereas 23 of these patients were found positive by XD-PCR (P=0.0016). Thus, not only was XD-PCR more sensitive than XD-M but it was also quicker, revealing more cases within 30 days than detected using XD-M over a period of 90 days. © 2007 The Liverpool School of Tropical Medicine.

Más información

Título según WOS: The PCR-based detection of Trypanosoma cruzi in the faeces of Triatoma infestans fed on patients with chronic American trypanosomiasis gives higher sensitivity and a quicker result than routine xenodiagnosis
Título según SCOPUS: The PCR-based detection of Trypanosoma cruzi in the faeces of Triatoma infestans fed on patients with chronic American trypanosomiasis gives higher sensitivity and a quicker result than routine xenodiagnosis
Título de la Revista: Annals of Tropical Medicine and Parasitology
Volumen: 101
Número: 8
Editorial: Taylor & Francis
Fecha de publicación: 2007
Página de inicio: 673
Página final: 679
Idioma: English
URL: http://www.maneyonline.com/doi/abs/10.1179/136485907X241415
DOI:

10.1179/136485907X241415

Notas: ISI, SCOPUS