Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets

De Diego M.; Avello,M.; Mennickent S.; Fernandez, M. ; Fernández P.

Abstract

In the present study, an RP high performance liquid chromatographic method was developed and validated for the determination of allicin in garlic powder and tablets. Chromatographic separation was carried out on an RP-18 e column (125 mm × 4 min), using a mobile phase, consisting of methanol-water (50:50 v/v), at a flow rare of 0.5 mL/min and UV detection at 220 nm. Ethylparaben was used as the internal standard. The assay was linear for allicin concentrations of 5.0-60.0 μg/mL. The RSD for precision was <6.14%. The accuracy was above 89.11%, The detection and quantification limits were 0.27 and 0.81 μg/ mL, respectively. This method was used to quantify allicin in garlic powder samples. The results showed that the method described here is useful for the determination of allicin in garlic powder and tablets. © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Más información

Título según WOS: Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets
Título según SCOPUS: Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets
Título de la Revista: JOURNAL OF SEPARATION SCIENCE
Volumen: 30
Número: 16
Editorial: WILEY-V C H VERLAG GMBH
Fecha de publicación: 2007
Página de inicio: 2703
Página final: 2707
Idioma: English
URL: http://doi.wiley.com/10.1002/jssc.200700161
DOI:

10.1002/jssc.200700161

Notas: ISI, SCOPUS