Dynein light-chain dynlrb2 is essential for murine leukemia virus traffic and nuclear entry

Pietrantoni, Gianfranco; Gaete-Argel, Aracelly; Herrera-Rojo, Diego; Ibarra-Karmy, Rodrigo; Bustos, Fernando J.; Valiente-Echeverria, Fernando; Arriagada, Gloria


Copyright © 2021 American Society for Microbiology. All Rights Reserved.Murine leukemia virus (MLV) requires the infected cell to divide to access the nucleus to integrate into the host genome. It has been determined that MLV uses the microtubule and actin network to reach the nucleus at the early stages of infection. Several studies have shown that viruses use the dynein motor protein associated with microtubules for their displacement. We have previously reported that dynein light-chain roadblock type 2 (Dynlrb2) knockdown significantly decreases MLV infection compared to nonsilenced cells, suggesting a functional association between this dynein light chain and MLV preintegration complex (PIC). In this study, we aimed to determine if the dynein complex Dynlrb2 subunit plays an essential role in the retrograde transport of MLV. For this, an MLV mutant containing the green fluorescent protein (GFP) fused to the viral protein p12 was used to assay the PIC localization and speed in cells in which the expression of Dynlrb2 was modulated. We found a significant decrease in the arrival of MLV PIC to the nucleus and a reduced net speed of MLV PICs when Dynlrb2 was knocked down. In contrast, an increase in nuclear localization was observed when Dynlrb2 was overexpressed. Our results suggest that Dynlrb2 plays an essential role in MLV retrograde transport. IMPORTANCE Different viruses use different components of cytoplasmic dynein complex to traffic to their replication site. We have found that murine leukemia virus (MLV) depends on dynein light-chain Dynlrb2 for infection, retrograde traffic, and nuclear entry. Our study provides new information regarding the molecular requirements for retrograde transport of MLV preintegration complex and demonstrates the essential role of Dynlrb2 in MLV infection.

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Título según WOS: Dynein light-chain dynlrb2 is essential for murine leukemia virus traffic and nuclear entry
Título de la Revista: JOURNAL OF VIROLOGY
Volumen: 95
Número: 15
Fecha de publicación: 2021


Notas: ISI