A semiempirical approach to the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer pathways in phycobilisomes

Matamala, AR; Almonacid, DE; Figueroa MF; Martinez-Oyanedel, J; Bunster, MC

Abstract

A semiempirical methodology to model the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer (FRET) pathways in the rods of the phycobilisomes (PBSs) from Fremyella diplosiphon is presented. Using the Förster formulation of FRET and combining experimental data and PM3 calculation of the dipole moments of the aromatic portions of the chromophores, transfer constants between pairs of chromophores in the phycocyanin (PC) structure were obtained. Protein docking of two PC hexamers was used to predict the optimal distance and axial rotation angle for the staked PCs in the PBSs' rods. Using the distance obtained by the docking process, transfer constants between pairs of chromophores belonging to different PC hexamers were calculated as a function of the angle of rotation. We show that six preferential FRET pathways within the PC hexameric ring and 15 pathways between hexamers exist, with transfer constants consistent with experimental results. Protein docking predicted the quaternary structure for PCs in rods with inter-phycocyanin distance of 55.6 A and rotation angle of 20.5°. The inter-phycocyanin FRET constant between chromophores at positions β155 is maximized at the rotation angle predicted by docking revealing the crucial role of this specific inter-phycocyanin channel in defining the complete set of FRET pathways in the system. © 2007 Wiley Periodicals, Inc.

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Título según WOS: A semiempirical approach to the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer pathways in phycobilisomes
Título según SCOPUS: A semiempirical approach to the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer pathways in phycobilisomes
Título de la Revista: JOURNAL OF COMPUTATIONAL CHEMISTRY
Volumen: 28
Número: 7
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2007
Página de inicio: 1200
Página final: 1207
Idioma: English
URL: http://doi.wiley.com/10.1002/jcc.20628
DOI:

10.1002/jcc.20628

Notas: ISI, SCOPUS