TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
Abstract
Tumor necrosis factor (TNF)-alpha is a pleiotropic cytokine implicated in the etiology of several autoimmune diseases, including rheumatoid arthritis (RA). TNF-alpha regulates diverse effector functions through the activation of TNF-alpha receptor (TNFR)1 and TNFR2. Although the detrimental role of this cytokine has been addressed in distinct disease settings, the effects of TNF-alpha on cytokine production by isolated CD4(+) T helper type 1 (Th1) and Th17 cells, two T cell subpopulations that contribute to the pathogenesis of RA, have not been completely elucidated. Here, we show that TNF-alpha promotes a reduction and expansion in the frequency of both T cell subsets producing IFN-gamma and IL-17, respectively. Selective blockade of TNFR1 or TNFR2 on Th1 and Th17 cells revealed that TNFR2 mediates the decrease in IFN-gamma production, while signaling through both receptors augments IL-17 production. We also demonstrate that Th1, but not Th17 cells from RA patients present lower levels of TNFR1 compared to healthy controls, whereas TNFR2 expression on both T cell types is similar between patients and controls. Since TNF-alpha receptors levels in RA patients are not significantly changed by the therapeutic blockade of TNF-alpha, we propose that targeting TNFR2 may represent an alternative strategy to normalize the levels of key cytokines that contribute to RA pathogenesis.
Más información
Título según WOS: | ID WOS:000846617100001 Not found in local WOS DB |
Título de la Revista: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
Volumen: | 23 |
Número: | 16 |
Editorial: | MDPI |
Fecha de publicación: | 2022 |
DOI: |
10.3390/ijms23169306 |
Notas: | ISI |