beta-Carotene Increases Activity of Cytochrome P450 2E1 during Ethanol Consumption

Sandoval, Cristian; Mella, Luciana; Godoy, Karina; Adeli, Khosrow; Farias, Jorge


One of the key routes through which ethanol induces oxidative stress appears to be the activation of cytochrome P450 2E1 at different levels of ethanol intake. Our aim was to determine if oral beta-carotene intake had an antioxidant effect on CYP2E1 gene expression in mice that had previously consumed ethanol. C57BL/6 mice were used and distributed into: control (C), low-dose alcohol (LA), moderate-dose alcohol (MA), beta-carotene (B), low-dose alcohol+beta-carotene (LA + B), and moderate-dose alcohol+beta-carotene (MA + B). Animals were euthanized at the end of the experiment, and liver tissue was taken from each one. CYP2E1 was measured using qPCR to detect liver damage. The relative expression level of each RNA was estimated using the comparative threshold cycle (Ct) technique (2(-Delta Delta CT) method) by averaging the Ct values from three replicates. The LA+B (2267 +/- 0.707) and MA+B (2.307 +/- 0.384) groups had the highest CYP2E1 fold change values. On the other hand, the C (1.053 +/- 0.292) and LA (1.240 +/- 0.163) groups had the lowest levels. These results suggest that ethanol feeding produced a fold increase in CYP2E1 protein in mice as compared to the control group. Increased CYP2E1 activity was found to support the hypothesis that beta-carotene might be dangerous during ethanol exposure in animal models. Our findings imply that beta-carotene can increase the hepatic damage caused by low and high doses of alcohol. Therefore, the quantity of alcohol ingested, the exposure period, the regulatory mechanisms of alcoholic liver damage, and the signaling pathways involved in the consumption of both alcohol and antioxidant must all be considered.

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Título según WOS: ID WOS:000801427700001 Not found in local WOS DB
Título de la Revista: ANTIOXIDANTS
Volumen: 11
Número: 5
Editorial: MDPI
Fecha de publicación: 2022


Notas: ISI