Expression of proinflammatory cytokines in osteoarthritis of the temporomandibular joint
Objective: This study reports the expression of proinflammatory cytokines in temporomandibular joint (TMJ) of patients affected with temporomandibular osteoarthritis (OA). Design: In twelve OA of the TMJ (OA-TMJ) affected patients and in six healthy volunteer subjects studied as control, the expression of IL1Î² (interleukin-1Î²), IL2, IL4, IL5, IL6, IL10, IL12p35, IL12p40, IL17, IFNÎ³ (interferon-gamma), TNFÎ± (tumor necrosis factor-alpha), and TNFÎ² mRNAs was evaluated. Using quantitative real-time RT-PCR technique, the cytokine levels, reported as Ct (cycle threshold), Î”Ct (Ct cytokine - Ct 18S rRNA) and RQ (relative quantification), in patient and control groups were compared. Results: Expression of IL1Î², IL2, IL12p35, IL12p40, IL17, TNFÎ±, TNFÎ², and IFNÎ³ mRNAs was significantly higher in patients as compared with controls. In particular, IL12 was the predominant cytokine expressed in patients (IL12p35 RQ = 30.2 and IL12p40 RQ = 29.0). Conversely, IL10 mRNA levels were higher in controls (RQ = 1.8). Conclusions: These data suggest that not only IL1Î², IFNÎ³, and TNFÎ± but also IL10, IL12, and IL17 are involved in the OA-TMJ pathogenesis. Furthermore, an inflammatory response characterised by the predominant expression of IL12 mRNA and down-regulated expression of IL10 mRNA is associated with the degenerative changes observed in OA-TMJ. Â© 2008 Elsevier Ltd. All rights reserved.
|Título según WOS:||Expression of proinflammatory cytokines in osteoarthritis of the temporomandibular joint|
|Título según SCOPUS:||Expression of proinflammatory cytokines in osteoarthritis of the temporomandibular joint|
|Título de la Revista:||ARCHIVES OF ORAL BIOLOGY|
|Editorial:||PERGAMON-ELSEVIER SCIENCE LTD|
|Fecha de publicación:||2008|
|Página de inicio:||910|