Temporal and spatial expression pattern of the myostatin gene during larval and juvenile stages of the Chilean flounder (Paralichthys adspersus)

Delgado, I; Fuentes E.; Escobar, S; Navarro, C.; Corbeaux, T; Reyes, AE; Vera, MI; Alvarez M.; Molina A.

Abstract

The full length cDNA sequence of the myostatin gene was cloned from a teleostean fish, the Chilean flounder (Paralichthys adspersus) through RT-PCR amplification coupled with the RACE approach to complete the 5′- and 3′-region. The deduced amino acid sequence encodes a protein of 377 amino acid residues, including the structural domains responsible for its biological activity. Amino acid sequence comparison revealed high sequence conservation, and confirmed that the isolated sequence corresponds to the MSTN1 gene. Gene expression analysis showed that cfMSTN mRNA is present in a wide variety of tissues in juvenile fish. In addition, we assessed the spatial expression pattern of the MSTN mRNA during embryos and larval stages through whole mount in situ hybridization. No expression was observed in embryos, whereas in larvae of 8 and 9 days post fertilization, the notochord, somites, intestine and some discrete territories in the head, such as brain and eye, were positive for MSTN mRNA. Our results contribute to the knowledge of the MSTN system in larval and juvenile stages; in particular the strong expression observed in the notochord suggests that MSTN, in synchronization with positive growth signals, may play an important role in the control of the development of larvae somites. © 2008 Elsevier Inc. All rights reserved.

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Título según WOS: Temporal and spatial expression pattern of the myostatin gene during larval and juvenile stages of the Chilean flounder (Paralichthys adspersus)
Título según SCOPUS: Temporal and spatial expression pattern of the myostatin gene during larval and juvenile stages of the Chilean flounder (Paralichthys adspersus)
Título de la Revista: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY MOLECULAR BIOLOGY
Volumen: 151
Número: 2
Editorial: Elsevier Science Inc.
Fecha de publicación: 2008
Página de inicio: 197
Página final: 202
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S1096495908001668
DOI:

10.1016/j.cbpb.2008.07.003

Notas: ISI, SCOPUS