Effect of the Availability of the Source of Nitrogen and Phosphorus in the Bio-Oxidation of H2S by Sulfolobus metallicus.

Silva, J.; Ortiz-Soto, R.; Morales, M.; Aroca, G.

Keywords: phosphorus, nitrogen, sulfolobus metallicus, nutrients limitation


first_pagesettingsOrder Article Reprints Open AccessArticle Effect of the Availability of the Source of Nitrogen and Phosphorus in the Bio-Oxidation of H2S by Sulfolobus metallicus by Javier Silva 1,*ORCID,Rodrigo Ortiz-Soto 1ORCID,Marjorie Morales 2 andGermán Aroca 3 1 Escuela de Ingeniería Química, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2162, Valparaíso 2340025, Chile 2 Department of Energy and Process Engineering, Faculty of Engineering, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway 3 Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2340025, Chile * Author to whom correspondence should be addressed. Fermentation 2023, 9(5), 406; https://doi.org/10.3390/fermentation9050406 Received: 6 March 2023 / Revised: 21 April 2023 / Accepted: 22 April 2023 / Published: 23 April 2023 (This article belongs to the Special Issue Application of Extremophiles in Biological Degradation and Conversion) Download Browse Figures Review Reports Versions Notes Abstract The effect of nitrogen and phosphorus availability on the growth of Sulfolobus metallicus was analyzed. This archaeon was subjected to a series of nitrogen and phosphorus limitation conditions to determine their effects on growth. The results indicate that Sulfolobus metallicus showed a relationship between one of the intermediate oxidation products (tetrathionate) and cell concentration during the exponential growth phase in the absence of nitrogen. Furthermore, significant differences were found in the specific growth rates under different scenarios with ammonia and phosphorus limitation, with values of 0.048 h−1 in the ammonia limitation case. The biomass substrate yield obtained was 0.107 gcel·g S−1. Meanwhile, in the absence of phosphorus, the specific growth rate was 0.017 h−1, and the substrate to biomass yield was 0.072 gcel·g S−1. The results indicate that the ability of Sulfolobus metallicus to bio-oxidize H2S depends on the availability of such nutrients (nitrogen and phosphorus), which affect cellular growth and the types of products generated. This, in turn, influences the oxidation process of various sulfur compounds, resulting in changes in the predominant products formed and the final oxidation of sulfate ions.

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Título de la Revista: FERMENTATION-BASEL
Volumen: 9
Número: 406
Editorial: MDPI
Fecha de publicación: 2023
Página de inicio: 406
Página final: 406
Idioma: English
URL: https://www.mdpi.com/2311-5637/9/5/406