A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells

Urra, J; Sandoval M.; Cornejo I.; Barros, LF; Sepulveda, FV; Cid, LP

Abstract

Extracellular pH, especially in relatively inaccessible microdomains between cells, affects transport membrane protein activity and might have an intercellular signaling role. We have developed a genetically encoded extracellular pH sensor capable of detecting pH changes in basolateral spaces of epithelial cells. It consists of a chimerical membrane protein displaying concatenated enhanced variants of cyan fluorescence protein (ECFP) and yellow fluorescence protein (EYFP) at the external aspect of the cell surface. The construct, termed pHCECSensor01, was targeted to basolateral membranes of Madin-Darby canine kidney (MDCK) cells by means of a sequence derived from the aquaporin AQP4. The fusion of pH-sensitive EYFP with pH-insensitive ECFP allows ratiometric pH measurements. The titration curve of pHCECSensor01 in vivo had a pK a value of 6.5±0.04. Only minor effects of extracellular chloride on pHCECSensor01 were observed around the physiological concentrations of this anion. In MDCK cells, the sensor was able to detect changes in pH secondary to H+ efflux into the basolateral spaces elicited by an ammonium prepulse or lactate load. This genetically encoded sensor has the potential to serve as a noninvasive tool for monitoring changes in extracellular pH microdomains in epithelial and other tissues in vivo. © 2008 Springer-Verlag.

Más información

Título según WOS: A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells
Título según SCOPUS: A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells
Título de la Revista: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volumen: 457
Número: 1
Editorial: Springer
Fecha de publicación: 2008
Página de inicio: 233
Página final: 242
Idioma: English
URL: http://link.springer.com/10.1007/s00424-008-0497-2
DOI:

10.1007/s00424-008-0497-2

Notas: ISI, SCOPUS