Two birds with one stone: integrating exfoliation and immunoaffinity properties in multi-walled carbon nanotubes by non-covalent functionalization with human immunoglobulin G

Mujica, Michael Lopez; Tamborelli, Alejandro; Vaschetti, Virginia M.; Espinoza, L. Carolina; Bollo, Soledad; Dalmasso, Pablo R.; Rivas, Gustavo A.


An innovative strategy is proposed to simultaneously exfoliate multi-walled carbon nanotubes (MWCNTs) and generate MWCNTs with immunoaffinity properties. This strategy was based on the non-covalent functionalization of MWCNTs with human immunoglobulin G (IgG) by sonicating 2.5 mg mL(-1) MWCNTs in 2.0 mg mL(-1) IgG for 15 min with sonicator bath. Impedimetric experiments performed at glassy carbon electrodes (GCE) modified with the resulting MWCNT-IgG nanohybrid in the presence of anti-human immunoglobulin G antibody (Anti-IgG) demonstrated that the immunoglobulin retains their biorecognition properties even after the treatment during the MWCNT functionalization. We proposed, as proof-of-concept, two model electrochemical sensors, a voltammetric one for uric acid quantification by taking advantages of the exfoliated MWCNTs electroactivity (linear range, 5.0 x 10(-7) M - 5.0 x 10(-6) M; detection limit, 165 nM) and an impedimetric immunosensor for the detection of Anti-IgG through the use of the bioaffinity properties of the IgG present in the nanohybrid (linear range, 5-50 mu g mL(-1); detection limit, 2 mu g mL(-1)).

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Título según WOS: ID WOS:000922845100003 Not found in local WOS DB
Título de la Revista: MICROCHIMICA ACTA
Volumen: 190
Número: 2
Fecha de publicación: 2023


Notas: ISI