Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose

Isachenko E.; Isachenko V.; Weiss, JM; Kreienberg, R; Katkov, II; Schulz, M.; Lulat, AGMI; Risopatron, MJ; Sanchez R.

Abstract

This study investigates the ability of sucrose to protect spermatozoa against mitochondrial damage, artificial cryoinduction of capacitation, and acrosome reaction. Spermatozoa were isolated using the swim-up procedure performed using three different media: (a) human tubal fluid (HTF, control) medium; (b) HTF with 1% human serum albumin (HSA); and (c) HTF with 1% HSA and 0.25 M sucrose. From each group, 30 λ suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by quickly submerging the spheres in HTF with 1% HSA at 37 °C with gentle agitation. Sperm motility, viability, mitochondrial membrane potential integrity, spontaneous capacitation, and acrosome reaction were investigated. Sperm viability, acrosome reaction, and capacitation were detected using the double fluorescence chlortetracycline-Hoechst 33258 staining technique. Mitochondrial function was evaluated using a unique fluorescent cationic dye, 5,5',6,6'-tetrachloro-1 -l',3,3'-tetraethyl-benzamidazolocarbocyanin iodide, commonly known as JC-1. The number of progressively motile spermatozoa was significantly higher in the sucrose-supplemented medium group (57.1 ± 3.2 %, P< 0.05) when compared with controls (119.4 ± 1.9%). The combination of HSA and sucrose (65.2 ± 2.6%) has a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P< 0.05) compared with HSA alone (32.6 ± 4.7%). In conclusion, vitrification of human spermatozoa with non-permeable cryoprotectants such as HSA and sucrose can effectively cryopreserve the cells without significant loss of important physiological parameters. © 2008 Society for Reproduction and Fertility.

Más información

Título según WOS: Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose
Título según SCOPUS: Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose
Título de la Revista: REPRODUCTION
Volumen: 136
Número: 2
Editorial: BIO SCIENTIFICA LTD
Fecha de publicación: 2008
Página de inicio: 167
Página final: 173
Idioma: English
URL: http://www.reproduction-online.org/cgi/doi/10.1530/REP-07-0463
DOI:

10.1530/REP-07-0463

Notas: ISI, SCOPUS - ISI