Improvement of the operational stability of the Novozym 435 (R) biocatalyst in the oxidation of 2,5-furandicarboxaldehyde to 2,5-furandicarboxylic acid in a continuous packed-bed microbioreactor
The present work reports the evaluation of the yield, productivity and operational stability of the chemoenzymatic oxidation of 2,5-furandicarboxaldehyde (DFF) to 2,5-furan dicarboxylic acid (FDCA) catalyzed by immobilized lipase B from Candida antarctica (Novozym (R) 435) in a continuous packed bed microbioreactor. The highest yields and productivities were achieved using a feed for the reaction medium at 36 mu L/min flow. The yield and specific productivity in continuous packed bed microbioreactor were approximately 20% and 1000 times higher than that obtained by operating in millibioreactors. The operational stability of the biocatalyst in the microbioreactor was higher than that achieved in a millibioreactor in continuous operation for 24 h. Finally, the packed bed microbioreactor is an interesting and straightforward strategy to increase the stability of the biocatalyst in continuous mode. (c) 2023 Elsevier Ltd. All rights reserved.
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