SIMULTANEOUS DETERMINATION OF ACETAMINOPHEN AND TRAMADOL BY SECOND DERIVATIVE SPECTROPHOTOMETRY

Toral, MI; Rivas J.; Saldias, M; Soto C.; Orellana, S

Abstract

"A rapid method for the simultaneous determination of acetaminophen (A) and tramadol (T) by second derivative spectrophotometric has been developed. From a solvent effect studies and the spectral behaviours of A and T, ethanol was selected as solvent. For a Δλ value of 210 nm a smoothing factor of 8,000 and scale factor of 1,000,000 were selected, because in these conditions the signal/noise ratio was favoured in order to avoid error. In these conditions also it is possible the simultaneous determination of A/T in a molar relation of 17/1 contained in pharmaceutical formulations. At 285.7 nm the second derivative value is T concentration dependent, corresponding to zero-crossing point of A. On the other hand, T does not absorb between 296.0 to 400.0 nm, thus 308.0 nm was selected for A determination by graphic method. The determination ranges for A and T were 8.1x10-7 - 5 1x10-5 mol/L and 3.4 x10-7 - 5.0 x10-5 mol/L, respectively and can be determined with good precision and accuracy, without previous separation. A study of interferents, was carried out by using the excipients Zafin

Más información

Título según WOS: Simultaneous determination of acetaminophen and tramadol by second derivative spectrophotometry
Título según SCIELO: SIMULTANEOUS DETERMINATION OF ACETAMINOPHEN AND TRAMADOL BY SECOND DERIVATIVE SPECTROPHOTOMETRY
Título de la Revista: Journal of the Chilean Chemical Society
Volumen: 53
Número: 2
Editorial: Sociedad Chilena de Química
Fecha de publicación: 2008
Página de inicio: 1543
Página final: 1547
Idioma: English
DOI:

10.4067/S0717-97072008000200022

Notas: ISI, SCIELO