Wnt-7a modulates the synaptic vesicle cycle and synaptic transmission in hippocampal neurons

Cerpa, W.; Godoy JA; Alfaro I.; Farlas, GG; Metcalfe MJ; Fuentealba, R; Bonansco, C.; Inestrosa, NC

Abstract

Wnt signaling is essential for neuronal development and the maintenance of the developing nervous system. Recent studies indicated that Wnt signaling modulates long term potentiation in adult hippocampal slices. We report here that different Wnt ligands are present in hippocampal neurons of rat embryo and adult rat, including Wnt-4, -5a, -7a, and -11. Wnt-7a acts as a canonical Wnt ligand in rat hippocampal neurons, stimulates clustering of presynaptic proteins, and induces recycling and exocytosis of synaptic vesicles as studied by FM dyes. Wnt-3a has a moderate effect on recycling of synaptic vesicles, and no effect of Wnt-1 and Wnt-5a was detected. Electrophysiological analysis on adult rat hippocampal slices indicates that Wnt-7a, but not Wnt-5a, increases neurotransmitter release in CA3-CA1 synapses by decreasing paired pulse facilitation and increasing the miniature excitatory post-synaptic currents frequency. These results indicate that the presynaptic function of rat hippocampal neurons is modulated by the canonical Wnt signaling. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.

Más información

Título según WOS: Wnt-7a modulates the synaptic vesicle cycle and synaptic transmission in hippocampal neurons
Título según SCOPUS: Wnt-7a modulates the synaptic vesicle cycle and synaptic transmission in hippocampal neurons
Título de la Revista: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 283
Número: 9
Editorial: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Fecha de publicación: 2008
Página de inicio: 5918
Página final: 5927
Idioma: English
URL: http://www.jbc.org/cgi/doi/10.1074/jbc.M705943200
DOI:

10.1074/jbc.M705943200

Notas: ISI, SCOPUS