The salivary peptide histatin-1 enhances bone repair in vivo

Torres, Pedro; Flores, Victor; Flores, Tania; Silva, Patricio; Gonzalez, Luis; Cordova, Luis A.; Reyes, Montserrat; Torres, Vicente A.

Abstract

The salivary peptide histatin-1 was recently described as a novel osteogenic factor that stimulates cell adhesion, migration, and differentiation in bone-lineage cells. Since these cell responses collectively contribute to bone regeneration, we hypothesized that histatin-1 harbors the capacity to enhance bone tissue repair at the preclinical level. By using a model of monocortical bone defect, we explored the effects of histatin-1 in tibial mineralization and organic matrix formation in vivo. To this end, different amounts of histatin-1 were embedded in one-mm3 collagen sponges and then applied to tibial monocortical defects in C57bl/6 mice. After seven days, mice were euthanized, and samples were processed for subsequent analysis. Micro-computed tomography screening showed that histatin-1 increased intra-osseous mineralization, and this phenomenon was accompanied by augmented collagen matrix deposition and closure of cortical defect edges, as determined by Hematoxylin-Eosin and Masson's Trichrome staining. Moreover, immunohistochemical analyses showed that histatin-1 increased the expression of the osteogenic marker alkaline phosphatase, which was accompanied by augmented blood vessel formation. Collectively, our findings show that histatin-1 itself promotes bone regeneration in an orthotopic model, proposing this molecule as a therapeutic candidate for use in bone regenerative medicine.

Más información

Título según WOS: ID WOS:001061841500001 Not found in local WOS DB
Título de la Revista: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volumen: 676
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2023
Página de inicio: 207
Página final: 212
DOI:

10.1016/j.bbrc.2023.07.047

Notas: ISI