Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxildae)

Jara Seguel, P.; Valdebenito I.; Palma Rojas, C.; Rebolledo C.

Abstract

The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp). This C value is within the range recorded for other osmeriform species (0.62-3.2 pg). The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard), which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.

Más información

Título según WOS: Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxildae)
Título según SCOPUS: Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
Título de la Revista: LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
Volumen: 36
Número: 1
Editorial: Facultad de Recursos Naturales. Escuela de Ciencias del Mar, Pontificia Universidad Católica de Valparaíso
Fecha de publicación: 2008
Página de inicio: 87
Página final: 91
Idioma: English
URL: http://www.lajar.cl/pdf/imar/v36n1/art07.pdf
DOI:

10.3856/vol36-issue1-fulltext-7

Notas: ISI, SCOPUS