Regulatory volume decrease in cardiomyocytes is modulated by calcium influx and reactive oxygen species

Rojas-Rivera, D; Diaz-Elizondo, J; Parra, V; Salas, D.; Contreras, A; Toro, B; Chiong M.; Olea Azar C.; Lavandero S.

Abstract

We investigated the role of Ca2+ in generating reactive oxygen species (ROS) induced by hyposmotic stress (Hypo) and its relationship to regulatory volume decrease (RVD) in cardiomyocytes. Hypo-induced increases in cytoplasmic and mitochondrial Ca2+. Nifedipine (Nife) inhibited both Hypo-induced Ca2+ and ROS increases. Overexpression of catalase (CAT) induced RVD and a decrease in Hypo-induced blebs. Nife prevented CAT-dependent RVD activation. These results show a dual role of Hypo-induced Ca2+ influx in the control of cardiomyocyte viability. Hypo-induced an intracellular Ca2+ increase which activated RVD and inhibited necrotic blebbing thus favoring cell survival, while simultaneously increasing ROS generation, which in turn inhibited RVD and induced necrosis. © 2009 Federation of European Biochemical Societies.

Más información

Título según WOS: Regulatory volume decrease in cardiomyocytes is modulated by calcium influx and reactive oxygen species
Título según SCOPUS: Regulatory volume decrease in cardiomyocytes is modulated by calcium influx and reactive oxygen species
Título de la Revista: FEBS LETTERS
Volumen: 583
Número: 21
Editorial: Wiley
Fecha de publicación: 2009
Página de inicio: 3485
Página final: 3492
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S001457930900773X
DOI:

10.1016/j.febslet.2009.10.003

Notas: ISI, SCOPUS