Regulation of inhibin/activin expression in rat early antral follicles

Andreone, L; Velasquez EV; Abramovich, D; Ambao, V; Loreti, N; Croxatto HB; Parborell, F; Tesone, M; Campo, S

Abstract

The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-aC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-aC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion. © 2009 Elsevier Ireland Ltd. All rights reserved.

Más información

Título según WOS: Regulation of inhibin/activin expression in rat early antral follicles
Título según SCOPUS: Regulation of inhibin/activin expression in rat early antral follicles
Título de la Revista: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volumen: 309
Número: 01-feb
Editorial: ELSEVIER IRELAND LTD
Fecha de publicación: 2009
Página de inicio: 48
Página final: 54
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S030372070900269X
DOI:

10.1016/j.mce.2009.05.003

Notas: ISI, SCOPUS