Development and validation of a reverse phase liquid chromatography method for the quantification of rasagiline mesylate in biodegradable PLGA microspheres

Fernandez, M. ; Barcia, E; Negro, S

Abstract

In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of rasagiline mesylate in biodegradable microspheres. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile:water (5:95, v/v) adjusted at pH 3.1. Flow rate was 1.0 ml min -1 and UV detection at 290 nm. Acyclovir was used as the internal standard. The calibration curve was linear over the range 0.5-20.0 µg ml -1. R.S.D. for precision was <1.8%. Accuracy ranged between 99.01% and 102.55% with a R.S.D. lower than 1.3%. LOD and LOQ were 0.07 µg ml -1 and 0.23 µg ml -1, respectively. The method was simple, rapid, and easy to apply, making it very suitable for routine analysis of rasagiline mesylate in biodegradable PLGA microspheres. It could be also used with reliability for the determination of the drug in other pharmaceutical dosage forms. © 2009 Elsevier B.V. All rights reserved.

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Título según WOS: Development and validation of a reverse phase liquid chromatography method for the quantification of rasagiline mesylate in biodegradable PLGA microspheres
Título según SCOPUS: Development and validation of a reverse phase liquid chromatography method for the quantification of rasagiline mesylate in biodegradable PLGA microspheres
Título de la Revista: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volumen: 49
Número: 5
Editorial: Elsevier
Fecha de publicación: 2009
Página de inicio: 1185
Página final: 1191
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0731708509001587
DOI:

10.1016/j.jpba.2009.02.031

Notas: ISI, SCOPUS